Analysis of distribution, phenotype and clinical correlation of CD163 positive macrophages in osteoarthritis / 中华疾病控制杂志

Objective The aim of this study was to analyze the distribution characteristics of CD163+ macrophages in peripheral blood and synovium of patients with osteoarthritis (OA)，and investigate the correlation of CD163+ macrophages, intracellular tumour necrosis factor(TNF-α), interleukin-10 (IL-10) expression with the disease duration, inflammation level and body mass index (BMI). Methods The percentages of CD163+ macrophages in synovium and peripheral blood and their intracellular levels of TNF-α and IL-10 were detected by flow cytometry. Meanwhile, immunofluorescence was uesed to observe the distribution of CD163+ macrophages in synovial tissue of patients with OA or fracture. Results (1) There were significant differences in the percentage of CD163+ macrophages in synovial and peripheral blood between OA and fracture patients(all P<0.05). (2) The expression of TNF-α in CD163+ macrophages of synovium and blood of OA patients with disease duration below 10 years was significantly higher than that of patients with OA disease duration above 10 years (all P<0.05) . The expression of IL-10 was significantly lower than that of patients with OA disease duration above 10 years (all P<0.05). (3) C-reactive protein (CRP) was positively correlated with BMI in patients with OA disease duration below 10 years (r=0.680， P<0.001), while showed no correlation with BMI in patients with OA duration above 10 years(r=0.084， P=0.187). (4) Immunofluorescence confocal microscopy showed a large number of CD163+ macrophages invaded around the synovial vessel of patients with OA. Conclusion The distribution of CD163+ macrophages in synovium and peripheral blood of OA patients with different course of disease was different, and showed differentiated cell subtypes.

IL-36 Promotes Inflammatory Activity and Inhibits Differentiation of Keratinocytes / 中国医学科学杂志(英文版)

Objective Psoriasis is an immune-mediated inflammatory disease. Despite advances in the study of its pathogenesis, the exact development mechanism of psoriasis remains to be fully elucidated. Hyperproliferative epidermis plays a crucial role in psoriasis. This study aimed to investigate the effects of interleukin-36 (IL-36) on keratinocyte dysfunction . Methods Human keratinocyte cell lines, HaCaT cells, were treated with 0 (control), 50 or 100 ng/ml IL-36 respectively for 24 h. Cell viability was determined with a cell counting kit-8 assay. Flow cytometry was used to assess the effects of IL-36 on apoptosis and cell cycle distribution. Expressions of the differentiation markers, such as keratin 10 and involucrin, were evaluated by quantitative real-time polymerase chain reaction (RT-qPCR). Expressions of the inflammatory cytokines, IL-1 and IL-6 were tested by ELISA. Results CCK8 assay showed the survival rate had no significant difference between the control and treated group ( > 0.05). Flow cytometry analysis showed cell cycle arrest at S phase in the IL-36-treated groups compared with the control group ( < 0.05). RT-qPCR verified the decreased mRNA expressions of keratin 10 and involucrin in the IL-36-treated groups compared with the negative control ( < 0.01). ELISA showed 100 ng/ml IL-36 enhanced levels of IL-1 and IL-6 in culture supernatants of HaCaT cells compared with the negative control ( < 0.05). Conclusion Taken together, these findings suggest that IL-36 could induce cell cycle arrest at S phase, inhibit keratin 10 and involucrin expressions and promote inflammatory activity in HaCaT cell lines.

Interleukin-6 in Psoriasis / 中国医学科学院学报

Psoriasis is a common,multifactorial,chronic inflammatory skin disease with an incompletely understood pathogenesis. A substantial number of inflammatory cytokines have been found to be elevated in psoriatic lesions,and the serum levels of a subset of these cytokines also correlate with the severity of psoriasis. Interleukin-6 is a multifunctional pro-inflammatory cytokine. Interleukin-6 is proved to be associated with many chronic inflammatory diseases and autoimmunity diseases such as psoriasis. This article reviews the relationship between interleukin-6 and psoriasis.

Classified and hierarchical storage technology for hospital medical data / 医疗卫生装备

Objective To explore hospital medical data so as to decrease the cost for medical information and construction while increase the efficiency of information system. Methods Hospital medical data were classified, and kinds of data layering technologies were applied to constructing the data center,and overall considerations and hierarchical arrangement were executed for types of medical data.The high efficiencies and high feasibility of business systems were realized to ensure data security. Results Classified and hierarchical storage technology enhanced business response and reliability with the same hardware infrastructure.Conclusion Types of technologies have to be combined according to the characteristics of the business system and its data so as to implement high-efficiency and-reliability storage of hospital medical data.

Homocysteine: A Potential Common Route for Cardiovascular Risk and DNA Methylation in Psoriasis / 中华医学杂志(英文版)

<p><b>OBJECTIVE</b>Homocysteine is a sulfur-containing amino acid with potential clinical significance. Abnormal homocysteine levels have been found in patients with psoriasis. This review summarizes the possible correlations among homocysteine, cardiovascular risk, and DNA methylation in psoriasis.</p><p><b>DATA SOURCES</b>We retrieved the articles published in English from the PubMed database up to January 2017, using the keywords including "psoriasis," "homocysteine," "cardiovascular risk," "DNA methylation," "methylenetetrahydrofolate reductase," "MTHFR," and "MTHFR C677T."</p><p><b>STUDY SELECTION</b>Articles about the roles of homocysteine in the cardiovascular risk and DNA methylation in psoriasis were obtained and reviewed.</p><p><b>RESULTS</b>Observational studies consistently reported that elevated homocysteine is an independent risk factor for cardiovascular diseases. Several studies also consistently reported an association between psoriasis and increased cardiovascular risk. A substantial body of evidence also suggested that an elevated homocysteine level is related to the demethylation of DNA. Data from clinical trials also demonstrated that MTHFR C677T polymorphisms as well as DNA methylation aberrations are associated with psoriasis.</p><p><b>CONCLUSIONS</b>This review highlighted the relationships among homocysteine, cardiovascular risk, and DNA methylation, suggesting that homocysteine may be a biological link between cardiovascular risk and DNA methylation in psoriasis.</p>

Inductive Effect of 5-Azacitidine on Apoptosis of Multiple Myeloma Cell lines and Its Mechanism / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the response of multiple myeloma (MM) cells to 5-azacitidine and its possible mechanisms.</p><p><b>METHODS</b>Two multiple myeloma-derived cell lines U266 and H929 were used in this study. The cell proliferation and apoptosis were assessed by CCK-8, flow cytometry and acridine orange staining. The cell cycle was assessed by flow cytometry. The expression of BCL-2, BAX was assessed by RT-PCR. The expressions of caspase-3 and p-ERK1/2 were assessed by Western blot.</p><p><b>RESULTS</b>The BCL-2/BAX ratio decreased, the activity of caspase-3 and p-ERK1/2 increased, the cell cycle was arrested in G2/M phase after treatment with 5-azacitidine. The 5-azacitidine inhibited proliferation in a dose-dependent manner.</p><p><b>CONCLUSION</b>5-azacitidine exerts apoptosis-inducing and grow-inhibiting effects on MM cell lines, its mechanism may be related with the decrease of BCL-2/BAX ratio, caspase-3 activation and the arrest of cell cycle.</p>

Role of SOX7 in Hematopoietic System Development and Hematological Malignancies--Review / 中国实验血液学杂志

The sex-determining region Y-box 7 (Sox7) is a important member of SOX family containing high mobi- lity group (HMG), mapped to human chromosome 8p23.1. Wnt/β-catenin signaling pathway plays an important role in cell survival, differentiation, self-renewal, proliferation and apoptosis, and is closely related with carcinogenesis. SOX7 gene is likely to be a tumor suppressor gene in MDS and other hematological malignancies. As a negative regulator of the WNT/β-catenin signaling pathway, the function loss of this gene can lead to carcinogenesis. The methylation of SOX7 gene leads to the silence of this gene, resulting in tumorigenesis. The decision of hematopoietic stem cells to self-renew or differentiate is a stochastic process, but SOX7 can promote the differentiation into all blood cell types. This review focuses on the role of SOX7 in hematopoietic system development and hematological malignancies.

Methylation Status of PTPL1 Gene in Non-Hodgkin's Lymphoma Cells / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To investigate the methylation status of PTPL1 in Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines, and the reversing effect of 5-Aza on expression of PTPL1 mRNA.</p><p><b>METHODS</b>The Hut78, Maver, Z138, CA46, Raji and Jurkat cell lines were cultured in vitro, the MS-PCR was used to detect the methylation status of PTPL1 gene and RT-PCR was used to detect the expression of PTPL1 mRNA, the CCK-8 method was used to determine the cell proliferation, the siRNA-mediated silencing of PTPL1 was used to clarify the role of PTPL1 in lymphoma cell lines.</p><p><b>RESULTS</b>PTPL1 gene promoter was non-methylated in Hut78, Maver and Z138 cells, and was hyper-methylated in Raji, CA46 and Jurkat cells; the different levels of PTPL1 mRNA expression were in Hut78, Maver and Z138; the re-expression of PTPL1 mRNA resulted from hyper-methylation was found in Raji, CA46 and Jurkat cells. The 5-Aza treatment could inhibit the prolifenation of Raji, CA46 and Jurkat cell lines and induce re-expression of PTPL1 mRNA. After PTPL1 was interfered by siRNA, the growth of lymphoma cells was promoted.</p><p><b>CONCLUSION</b>The PTPL1 gene silencing induced by hyper-methylation may be an important factor in the occurrence and development of non-Hodgkin lymphoma. The methylation of PTPL1 gene may be used as a possible molecular marker for diagnosis and treatment targets.</p>

Expression and Significance of PTPL1 in Hematological Malignancies / 中国实验血液学杂志

PTPL1 is a protein with a predicted MW of 270 kD, and plays a major role in many cellular functions, including cell survival, proliferation, differentiation and motility. Evidence has demonstrated that PTPL1 is associated with tumor. Although many conflicting results suggested that PTPL1 has two contradictory effects (supressing or promoting ) on tumor, the real effect depends on the involved substrate and the cellular context. Expression of PTPL1 is low in lymphoma, while it is high in myeloid leukemia. PTPL1 has been regarded as a tumor suppressor in lymphoma, the methylation of PTPL1 promoter leads to gene expression reduced or disappeared, playing a lymphoma tumor suppressor role. This review focuses on PTPL1 domain and its interacting proteins, the relationship between PTPL1 and hematological malignancies.

Studies on flavoniod constituents of Hedysarum multijugum / 药学学报

<p><b>AIM</b>To study the chemical constituents from Hedysarum multijugum.</p><p><b>METHODS</b>The compounds were separated by chromatography methods, their structures were identified by spectral analysis.</p><p><b>RESULTS</b>Six compounds were isolated and identified as beta-sitosterol (1), 7-hydroxy-4'-methoxy isoflavone (2), betulic acid (3), 1,7-dihydroxy-3, 9-dimethoxy pterocarpene (4), 5, 7-dihydroxy-8-C-prenyl-4'-methoxy isoflavone (5) and 5, 7-dihydroxy-4'-methoxy isoflavone (6).</p><p><b>CONCLUSION</b>Compound 4 is a new compound and the others were obtained from the plant for the first time.</p>